New signs have been placed, warning drivers that trucks with five axles or more are not allowed on Angeles Crest Highway, also known as Route 2.
"Sometimes the truck drivers, they don't mean no harm, but sometimes they don't see the regular drivers, and they can kind of be aggressive at times," said motorist Chuck Jefferson.
The ban comes after a truck transporting cars slammed into several vehicles and a bookstore, killing two people.
A memorial has been set up at the intersection of Foothill and Angeles Crest Highway.
The trucker, 43-year-old Marcos Costa from Massachusetts, apparently ignored other warning signs and used Angeles Forest Highway, which already bans semi-tractor trailers. The truck burned out its brakes on the steep grade.
Costa was arrested on suspicion of gross vehicular manslaughter and reckless driving.
The accident killed 12-year-old Angelina Posca and her father Angel Jorge Posca, both from Palmdale. Their red Ford sedan was pushed through the intersection by the truck and crushed.
A dozen other people, including the trucker, were injured. Three people were hurt critically.
The accident follows another truck accident in September 2008 when a runaway truck plowed through a parking lot in the same block, injuring one person.
Officials said the ban is a temporary one, but they are looking at ways to make it permanent.
Costa has been charged with two felony counts of vehicular manslaughter in connection with the crash. His arraignment was postponed until April 13.
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