Among the stars at the Scream fest, rock musician Marilyn Manson and cast members from the former ABC show "Lost" which was honored at the awards show.
Other famous names who graced the stage included Halle Berry, Megan Fox, Don Cheadle, Kelly Osbourne and Mickey Rourke.
"Twilight" took the prize for best fantasy film. Kristen Stewart, Nikki Reed and Jackson Rathbone accepted the award.
Fans voted online for awards in 30 categories, including best villain and most memorable mutilation. Not all 30 prizes were presented during Saturday's show.
In a sneak peak, Wes Craven, Neve Campbell, Emma Roberts and David Arquette presented footage from "Scream 4."
But the most anticipated movie, according to Spike viewers, was "Green Lantern." Stars Ryan Reynolds and Blake Lively appeared together to accept the night's final prize.
Though Saturday's show spanned more than three hours, "Scream 2010" is set to air Tuesday as a 90-minute special.
The Associated Press contributed to this report.