The slow-tempo pop song written by John and Bernie Taupin only features John in the film's soundtrack. The duet with Lady Gaga is only featured in the movie. The track will also be kept from being released over the radio, said a spokeswoman for Buena Vista Records.
John said he asked Lady Gaga to join him in the duet last year after she performed at a charity benefit at his home in England.
Lady Gaga is expected to perform the title track from her upcoming album, "Born This Way" on Feb. 13 at the Grammys, the same awards show where John joined Lady Gaga last year for a mash-up of her "Speechless" and his "Your Song."
"Gnomeo & Juliet" is scheduled to debut Feb. 11. The soundtrack is set for release Feb. 8.
The Associated Press contributed to this report.