Members of the Colonia Chiques gang were arrested for various drug-related offenses, including possession and sale.
Eight members were arrested on federal warrants; 14 others were arrested on state charges; and two gang members were still at large and actively being sought by authorities.
Twelve defendants are accused of distributing methamphetamine, crack cocaine and heroin.
The defendants were scheduled to be arraigned in U.S. District Court in L.A. Wednesday afternoon.
Most of the defendants named in the federal indictments face mandatory-minimum sentences of at least five years and statutory maximum sentences of 40 years in federal prison if convicted. Two defendants face potential life sentences because of prior convictions.
The joint investigation between the FBI and Oxnard Police, among several other federal and state agencies lasted eight months and culminated in Wednesday's arrests.