The girls, ages 4, 2 and 4 months, were locked naked in the room over the weekend.
John Robey, 33, and Christina Moore, 26, are accused of nailing drywall over the entrance. The 4-year-old managed to break free and get help from a neighbor.
Police say the room was covered in feces and urine, and believe the girls were locked inside for about a day.
All three seemed to be healthy and are currently in protective custody.