Fans watched in horror as the bus's front wheel rolled over the cup.
The 33-pound cup slipped from the hands of Madrid defender Sergio Ramos while atop the team bus during celebrations Thursday morning in the capital.
Emergency service workers gathered up the broken pieces and returned them to the bus. The trophy did not reappear at the club's traditional celebration spot at the Plaza de Cibeles in central Madrid.
The trophy will be repaired, and once it's put back together, it will go in the team's trophy case.
The Associated Press contributed to this report.