Defense attorneys filed the motion late Friday asking to immediately stay the trial and to reverse Judge Michael Pastor's order to not sequester the jurors. They argued that the case will attract immense media coverage and jurors should not be exposed to that.
Murray was in court for Tuesday's pre-trial hearing, but was not present Wednesday when attorneys finalized the juror questionnaire.
Pastor ruled on Wednesday that prosecutors can use a study showing that propofol needs to be injected to have any effect. Defense attorneys had objected to that study.
Murray is charged with involuntary manslaughter in connection to Jackson's 2009 death. He is accused of administering deadly amounts of the sedative propofol.
Murray was hired as Jackson's personal physician. Prosecutors say that he is criminally responsible for Jackson's death.
Defense attorneys say that Jackson administered the drug on his own.
The trial will go on as scheduled, with jury selection beginning Thursday at 9 a.m.