Demetrius Hewlin, who was wounded during a shooting at Chardon High School, died Tuesday morning, the hospital said in a statement.
"We are very saddened by the loss of our son and others in our Chardon community. Demetrius was a happy young man who loved life and his family and friends," his family said in a statement. "We will miss him very much, but we are proud that he will be able to help others through organ donation. We ask that you respect our privacy during this difficult time."
Russell King Jr. was declared brain dead just before 1 a.m. It was unclear whether King remained on life support; the statement referred to him as both deceased and brain dead.
King, 17, was studying at nearby Auburn Career Center and like the others who were shot was waiting for a bus for his daily ride to the center. Student Daniel Parmertor died hours after the shooting.
T.J. Lane allegedly opened fire in the school cafeteria during breakfast on Monday, hitting five students. He was taken in to custody shortly after the shooting.
Lane made his first court appearance on Tuesday. According to prosecutor David Joyce, Lane did not know the victims; he chose them randomly. Lane was ordered to remain in detention during proceedings due to the serious nature of the allegations. Lane has not yet been charged. The prosecution has until March 1 to formally charge him.
Judge Timothy Grendell banned lawyers from talking to any media outlet about the case or about Lane.
Police said the 17-year-old does not attend the school. He's a sophomore at an alternative school for troubled students. His friends say Lane was bullied for his Goth appearance.
"He was just a person who wanted to himself and keep all of his emotions in," said Lane's friend Jonathan Denoto. "They came out in a way that, in a way nobody would expect a kid like T.J. to come out as."
A student who saw the attack up close said it appeared that the gunman targeted a group of students sitting together and that the one who was killed was gunned down while trying to duck under the cafeteria table.
Classes were canceled on Tuesday, but grief counselors were on hand. Classes are set to resume on Friday.
Two students remain hospitalized. FBI officials would not comment on a motive.
The Associated Press contributed to this story.