Results from the U.K. Forensic Science Service showed corrosion deposits were considered "compatible with immersion in sea water," while a silver expert studied a plate on the violin's neck to determine if it fit the time profile.
Wallace Hartley was the Titanic's bandmaster, and survivors say he played the rosewood violin as the ship went down in 1912.
Hartley did not survive, but it's believed the violin was found strapped to his body and later returned to his grieving fiancée. It later ended up in the hands of the Salvation Army before being given to a violin teacher and ultimately auctioneers Henry Aldridge & Son.
The violin is expected to fetch at least $600,000 at auction.
The Associated Press contributed to this report.