Timberlake joins a growing list of performers that were previously announced, including Luke Bryan, Miley Cyrus, Lady Gaga, Imagine Dragons and One Direction.
The 32-year-old artist is currently on tour for "The 20/20 Experience - The Complete Experience."
Earlier this year, Timberlake became the sixth artist in Billboard history to have multiple No. 1 albums with a group and as a solo artist.
The 2013 American Music Awards will broadcast live from the Nokia Theatre at L.A. Live on Nov. 24. This year's host is recording artist Pitbull.