The chaotic scene was one of many around the country, where thousands took to the streets to protest Trump's stunning upset in Tuesday's presidential election.
Large crowds also gathered in San Francisco, Oakland, New York, Chicago, Boston, Philadelphia, Austin among other locations.
The demonstrations involved angry chants, anti-Trump signs and a few incidents of flag burning.
In Los Angeles, thousands of people first massed peacefully near City Hall, blocking one side of the street, holding signs and chanting "Not my president."
Later in the evening as the crowds grew to the thousands, they spilled out onto the 101 Freeway near downtown, shutting it down in one direction as police then blocked lanes on the other side.
Some motorists were stuck in their cars for hours, as protesters filed past them. By midnight, police were letting some of the stuck vehicles on the southbound side leave, but the freeway remain closed overall in both directions.
Police made at least 13 arrests as they began trying to stop more protesters from entering the freeway.
Protesters were also seen spray-painting downtown buildings as well as news vans with anti-Trump slogans.
They also burned a large pinata designed to look like Trump's head.
Anti-Trump crowd continues to grow at City Hall. Spring St. now shut down in both directions, well more than 1,500+ protesters. More to come pic.twitter.com/VE8Dw6c8iS— Jory Rand (@ABC7Jory) November 10, 2016
In New York City, crowds of several thousand marched in front of Trump Tower. One protester lit an American flag on fire as crowds cheered anti-Trump slogans.
American flags were also burned in Portland and at American University in Washington D.C.
There were similar protests held election night after it became clear that Trump had confounded the pollsters and delivered an upset victory, sweeping most of the battleground states against all expectations.
Hundreds of students at UCLA and UC Irvine took to the streets to protest the results.
Some violence was reported at the Oakland protest late Tuesday night, when some demonstrators set fires, broke windows and sprayed graffiti on downtown businesses.