It was a promotional event for Dreamworks' upcoming release "Megamind."
It took at least 1,501 superheroes of all ages to break the old record.
Ferrell, who stars in the animated feature, made a grand entrance and gave the crowd the good news.
"You and you alone have broken the Guinness Book of World Records today, right now, and everyone in a Spiderman outfit wins a new car! No? Oh okay, I was mistaken," Ferrell said.
"It's very impressive that people would come out and have a great time and it's a great kick-off for the movie," Ferrell added.
"Megamind" opens in theaters Nov. 5.
