Up on the auction block were 750 items from Wayne's life -- from costumes, scripts, awards, artwork and other memorabilia -- including the actor's last driver's license, a holster and gun belt from the film "El Dorado," and his Rooster Cogburn hat and eye patch from the 1969 movie "True Grit."
The green wool beret Wayne wore in "The Green Berets," went for $179,250 and a cowboy hat worn in "Big Jake" and "The Cowboys" fetched $119,500.
The items had been kept by Wayne's family since his death in 1979 from cancer.
A portion of the proceeds will be used by the John Wayne Cancer Foundation to help fund cancer research, treatment, and education.
The Associated Press contributed to this report.