There will be at least three key witnesses against Sandusky, who is charged with 40 criminal counts for alleged sexual abuse against eight boys over 15 years.
The victim claims Sandusky savagely raped him, according to his lawyer Ben Andreozzi.
"He is not going anywhere. He fully intends to testify," said Andreozzi.
Andreozzi said his client was outraged by Sandusky's portrayal of what happened to him as horseplay. The former coach claims he's innocent and said in a recent interview that he had showered with boys but never molested them.
"I think he would like to see Mr. Sandusky assume responsibility for the horrible acts that he committed on him," Andreozzi said.
The mother of alleged Victim 1, a boy who told the grand jury he was repeatedly sexually assaulted in Sandusky's basement, said her son is anxious to testify against the accused pedophile.
"He's ready. He wants to do it. He wants him to go to jail, and he wants him to pay for what he's done," said the mother.
The two boys will join Mike McQueary as the prosecution's main witnesses against Sandusky. According to court documents, McQueary said he witnessed Sandusky raping a 10-year-old boy in the Penn State locker room in 2002 and told head coach Joe Paterno.
In an email to friends, which was obtained by the Associated Press, McQueary said he also told police about what he saw. Both the campus police and the State College police said they have no record of McQueary reporting the alleged rape.
The witnesses will testify before a new judge in the Sandusky case. The previous judge was removed Wednesday after it was revealed she had donated money to The Second Mile, a charity founded by Sandusky for at-risk youth.
Sandusky is a married father of six adopted children, including Matt Sandusky. Matt's biological mother, Debra Long, expressed concern for her son, who began living at the coach's home in State College in 1995. She said Sandusky showered her son with presents and was unusually close to him.
"I think Jerry was buying the kid," she said. "He was kind of suckering him in to whatever."
While Long doesn't know if her son was sexually abused by Sandusky, she is not surprised by the charges he's facing.
"He's a liar. He's artistic in covering all of himself up," Long said.
Sandusky is free on bail, but he's due back in court Dec. 7.
Meanwhile, Pennsylvania state lawmakers are starting to plan for a special commission that will examine the legal issues raised by the child sex-abuse scandal. Topics are likely to include mandatory reporting of suspected abuse, and the legal definition of child abuse, officials said.
The Associated Press contributed to this report.