The collision occurred shortly after 6:45 p.m. at Kanan Dume and Latigo Canyon roads as the deputies were responding to a call about a person throwing bottles in the middle of Pacific Coast Highway.
No other vehicles were involved in the crash.
The two deputies were airlifted to a hospital and suffered non-life-threatening injuries.
The area remained shut down while officers conducted an investigation. Anyone with information is asked to call Lost Hills Station at (818) 878-1808.