The collision was reported at 3:38 a.m., prompting a SigAlert and the temporary closure of all southbound lanes on the freeway at Avenue M in Lancaster, according to the Highway Patrol.
Video from AIR7 HD showed southbound traffic backed up for miles amid the shutdown.
All lanes were reopened as of 7:40 a.m., the CHP announced.
The cause of the crash was not immediately known.
SIGALERT CANCELLATION: SB SR-14, NORTH OF AVE. M, ALL LANES OPEN— CHP PIO - LA County (@CHPsouthern) September 20, 2016