San Vicente Boulevard will be closed between Santa Monica Boulevard and Melrose Avenue starting at 7 p.m. Thursday and won't reopen until Monday morning.
Parts of Santa Monica Boulevard will also be closed through the weekend.
The June 10-12 event includes musical performers such as Carly Rae Jepsen and Charli XCX, as well as a festival and parade on Sunday.
The parade's grand marshal will be Jewel Thais-Williams, an activist who founded one of the area's first clubs for black LGBT people.