The overnight closures are expected to begin some time next month, but an exact date has not been announced.
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Construction continues for Wildlife Crossing over 101, which will offer safety for mountain lions
All lanes will be closed in only one direction between Liberty Canyon Road and Chesebro Road on weekdays. Lanes in the opposite direction will remain open for traffic.
Caltrans has not said which direction they will start with first. Traffic will be diverted to local streets and nearby freeways as crews install large concrete beams above the freeway lanes.
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The Wallis Annenberg Wildlife Crossing will span over 10 lanes of the 101 Freeway when completed, and aims to provide a connection between the small population of mountain lions in the Santa Monica Mountains and the larger and genetically diverse populations to the north.
It will be the largest crossing of its kind in the world, stretching 210 feet over the freeway. Completion of the crossing is expected at the end of next year or early 2026.
City News Service contributed to this report.