The Oscar-nominated movie will be shown at the famed venue on a giant screen as a live orchestra performs the score.
Fans of the movie can dance the night away with an all-women, mostly women of color orchestra known as the Barbie Land Sinfonietta.
Tony Award-winner Macy Schmidt will be the conductor, according to the Hollywood Bowl's website.
"Barbie The Movie: In Concert" will take place July 27.
Single tickets for the show go on sale May 7.
The Greta Gerwig-directed film was the highest grossing film of 2023 and is nominated for eight Academy Awards, including Best Picture.