Glendora police responded to a call about a bobcat around East Leadora Avenue just after 6 a.m.
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After responding, officers determined it was a mountain lion weighing about 100 pounds.
Officers tried to get the big cat back up into the hills, but he kept roaming, eventually jumping into a backyard and then climbing up a tree.
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The Department of Fish and Wildlife finally tranquilized the cat and relocated him back into the hills.