The video posted in December showed the lion cub sitting in the backseat with the convertible top open as the luxury car drives slowly past astonished onlookers.
This week the country's Central Investigation Bureau announced the woman that owns the lion has been charged with illegal wild animal possession.
She's facing up to a year in prison and a maximum fine of almost $3,000.
Local media reports indicate it is not illegal to privately own a lion in Thailand, but the woman allegedly failed to follow the proper government procedures and paperwork to acquire and register the exotic animal.