The male lion known as P-90 was killed by a car on Highway 33 just south of Ojai in Ventura County on Friday morning, according to officials with the Santa Monica Mountains National Recreation Area.
P-90 was just a little over 1 year old. He was first captured and tagged at the den in July 2020, when he was estimated to be around three weeks old.
His brother, P-89, who was about two years old, was killed by a car on the 101 Freeway in Woodland Hills last month.
RELATED: Mountain lion P-89 killed on 101 Freeway in Woodland Hills
Mountain lion P-89 dies after being struck by vehicle on 101 Freeway in Woodland Hills
Santa Monica Mountains officials say P-90 is the seventh tracked mountain lion to be killed on a SoCal road this year. Over the 20 years of an ongoing study, 32 have been killed by a vehicle in the area.
But the local mountain lion population also had some new additions recently.
Biologists recently visited and tagged two mountain lion kittens in a den while their mom was away. The kittens were given the identities P-107 and P-108. They were believed to be about 17 days old when the biologists visited.
Work is now underway on a wildlife crossing over the 101 Freeway in Agoura Hills, an effort that is aimed at helping wildlife move about the region safely.
Groundbreaking held for wildlife crossing over 101 Freeway
Groundbreaking for bridge over 101 Freeway, billed as world's largest wildlife crossing