The incident occurred after officers responded to the gas station in the 4300 block of East Ontario Mills Parkway around 10:45 p.m. Thursday, according to the Ontario Police Department.
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Authorities said the man had been threatening customers and a clerk with the knife, and was demanding money.
Responding officers opened fire after the suspect charged at police, police said.
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No officers or customers were injured. The suspect was pronounced dead at the scene.