The five pound coin features the band's logo and instruments played by the Queen members and pays tribute to "Bohemian Rhapsody."
On the flip side, if shows Queen Elizabeth II, as all British coins and banknotes bear the head of the monarch.
The coin is available in a gold proof, silver proof and a more affordable brilliant uncirculated coin.
"This is a 'Who would have thought it?' moment," said Brian May, the group's legendary guitarist.