Mayor Bill de Blasio said it is New York City's deadliest fire in two years.
The fire broke out in a house on 112-16 208th Street in Queens Village just after 2:30 p.m.
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The three-alarm fire took hours to get under control. No firefighters were seriously injured.
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FDNY Commissioner Daniel Nigro said the youngest victim of the fire is believed to be 2 years old and the oldest victim is believed to be 21 years old.
A 46-year-old man who came out of the second-floor window of the home was in fair condition.
No further information was released as an investigation into the cause continued.