The victim, who was shot in the arm, identified himself as an employee at Sony Pictures Studios, according to a spokesperson for the Culver City Police Department.
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Where on the 10 Freeway the shooting occurred was unclear, police said.
Video from AIR7 showed a man being placed on a gurney and loaded into an ambulance by firefighter-paramedics.
California Highway Patrol officers also responded to the studio lot and took over the investigation.
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A description of a possible suspect was not released.