The launch at Vandenberg Air Force Base was postponed Sunday to conduct additional inspections of the second stage, the third time the launch has been delayed.
SpaceX said families paid $2,500 to put a sample of their loved ones ashes on the rocket.
The ashes were onboard four-inch mini-satellites that families will be able to track from Earth using an app.
Falcon 9 also carried 64 satellites -- commercial and government entities from more than 17 countries -- on what's being called a "ridesharing" mission.
The launch is the 19th of the year for the Hawthorne-based company.