Hazmat teams were called to the scene between Laurel Canyon Boulevard and Coldwater Canyon Drive shortly before 8 a.m. following a traffic accident that caused the tanker to tip over.
It was carrying 500 gallons of water and 60 gallons of a substance often used as a soil fumigant, some of which spilled onto the roadway, according to the Los Angeles Fire Department. Though the spill was contained, transportation officials said the street would be closed for "the better part of the work day" as crews worked to clean up.
By 11 a.m., Mulholland Drive remained closed and an estimated time for reopening was not available.
The driver was taken to the hospital with minor injuries.