The incident allegedly happened on March 14 at the Kinder Kids Christian Preschool in Mid-Wilshire.
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The surveillance video shows the child on the floor as a worker grabs his arms. Then, she pins the boy on the ground.
The child's mother, BrieAnn Battle, couldn't believe what she was seeing when she saw the video.
"I was distraught," said Battle. "Nobody wants to see their child being manhandled like that at all."
The woman then grabs the boy by his ankles and carries him upside down to a table. Battle says the owner didn't tell her anything about this. She said it was an employee at the daycare who showed her the video.
"How dare ... you know, how dare you? And then she's avoiding accountability, so it's turned very criminal," said Battle.
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She says when she reached out to meet with the owner, she claims she was shown different video to hide what had happened.
"The owner-operator showed me the clip from when she took him out into the foyer, and that's when he was giving her a lot of activity so she thought that was going to be, I guess, a better angle," said Battle.
"I tried to even give her the benefit of the doubt when I went into meet with her, not excusing her behavior, but from mother-to-mother, woman-to-woman, things do get out of control," she continued. "She's denying everything, and it's sickening."
Eyewitness News asked the preschool for an interview or a statement about the incident. No one would speak with ABC7 and we did not hear back. Eyewitness News did speak with the employee in the video and said she has since resigned.
Battle has filed a police report, reporting the incident as child abuse.
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"For that matter, she doesn't need to be taken care of anybody else's kids, and her license needs to be revoked," said Battle. "She needs to go to jail for this."
Battle has also hired a lawyer. She says she does not want this happening to any other child.
The incident remains under investigation.