Tuesday night was slated to be the last night of closures for the southbound lanes of the 101 Freeway. Road work starts impacting northbound traffic on Wednesday.
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All northbound lanes on the 101 will close at Liberty Canyon Road starting Wednesday at 11:59 p.m.
To get around the closure, motorists can take the 405 Freeway to the 118 to the 23 -- or reverse if you're heading eastbound. Lanes are expected to reopen at 5 a.m.
The crossing will be one of the largest crossings of its kind in the world, stretching 210 feet over 10 lanes of the 101 Freeway.
It aims to provide a connection between the population of mountain lions and other wildlife to the larger and more genetically diverse populations to the north.
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MORE: Crews make steady progress on wildlife crossing over 101 Freeway
Crews make steady progress on wildlife crossing over 101 Freeway in Agoura Hills