The O'Neals were residents of Florida, but according to court documents, Shaunie O'Neal has been a resident of Los Angeles since Sunday, one day before the separation papers were filed.
According to court documents, she is seeking spousal and child support, as well as full legal custody of the couple's four children.
Two years ago, Shaq filed for divorce, but the couple reconciled.
The couple has been married for nearly seven years.