Police said Brooke Mueller was taken into custody early Saturday morning at a nightclub in Aspen after a woman reported being assaulted by her.
She posted $11,000 bail and is scheduled to be in court Dec. 19.
Her attorney had no comment.
Sheen and Mueller divorced earlier this year, citing Christmas Day 2009 as the day of their breakup. Charlie Sheen was arrested in Aspen on that day on suspicion of assaulting Mueller. He completed his probation in that case last November.
The Associated Press contributed to this report.