The women stayed at the Rodeway Inn-Regalodge Motel at 200 West Colorado Street for three nights in Oct. 2009.
Plaintiffs Nicole Eatman, Vera Domini and Regina Martocci claim the bed and parts of the furniture were filled with adult bed bugs, larvae and eggs.
The women are suing Rodeway Inn-Regalodge Motel and its franchiser, Choice Hotels International Inc., seeking restitution for medical bills and mental anguish.
The women's lawyer is claiming that the owners of the franchise knew they had a bed bug problem in room 212.
"In my opinion I never even thought of about bed bugs before having them. So it was totally not on my list of things to even be aware of," said Domini.
Choice Hotels International had no comment for Eyewitness News.
A jury trial is scheduled to begin May 2.
City News Service contributed to this report.