The promoter had been working with Michael Jackson on his comeback concerts when he died.
Mrs. Jackson's complaint accuses AEG Live of failing to honor its agreement to provide the doctor charged in the singer's death with life-saving equipment.
The suit claims AEG Live is liable for the actions of Dr. Conrad Murray, who has pleaded not guilty to involuntary manslaughter in the singer's death.
Mrs. Jackson, who is the guardian of Michael's three children, is also suing on their behalf.
She is seeking unspecified damages.
The suit also says AEG Live was aware of Michael's physical condition but cared more about profits than his health, and that the company's actions and inactions led to Michael's death.
AEG Live Spokesman Michael Roth says the company has not seen the lawsuit and has no immediate comment.
Conrad Murray wants a wrongful death lawsuit filed against him to be thrown out.
Lawyers for Murray filed a motion seeking dismissal of a suit filed by Michael Jackson's father, Joe.
They say Joe Jackson didn't have the legal right to file the lawsuit because he's not a beneficiary of the singer's estate.
Michael Jackson died in June 2009 at the age of 50.
In the case against AEG, Katherine Jackson wants a jury trial.