The 37-year-old gave no reason for the split, but the supermodel said she would focus on other projects.
"All good things have to come to an end. I will always love Victoria and never tell her secret," said Klum.
Last year, Klum walked the lingerie runway just five weeks after giving birth.
Meanwhile, the "Project Runway" host is also creating an activewear collection with New Balance, to be sold on Amazon.
The Associated Press contributed to this report.