This will be the Grammy Award-winning singer's second appearance at the NFL championship. Aguilera was part of the halftime show during the 2000 Super Bowl.
Stars like Carrie Underwood, Billy Joel, Diana Ross, Neil Diamond, Mariah Carey and Beyonce Knowles have sung the national anthem at the Super Bowl in the past.
The Pittsburgh Steelers will play the Green Bay Packers at Cowboys Stadium in Arlington, Texas, for the NFL title.
The Associated Press contributed to this report.