The truck driver and one person on the train were killed in the collision, which occurred shortly before 11:30 a.m. on U.S. 95 about 70 miles east of Reno, said authorities.
An unknown number of other people aboard the train were injured and were being taken by ambulances to area hospitals.
Amtrak spokesman Marc Magliari said most of the injuries were not life threatening, but he gave no other details about the injuries. Magliari said 204 passengers and 14 crew members were on the train en route from Chicago to Emeryville, Calif., which is about 300 miles west of the crash site.
The accident shut down a section of U.S. 95 between Interstate 80 and Fallon, Nev.
The Associated Press contributed to this report.