The last horse death was reported on Saturday. It happened during the third race of the day when Eskenforadrink suffered a life-ending injury to her ankle when she was leading the pack. The 4-year-old filly had to be euthanized.
The latest horse was put down following a leg injury during training. The park announced earlier Tuesday it had hired Dennis Moore, a consultant for the track.
The Stonach Group, which owns the park, said in a press release that racing and training were suspended while the 1-mile track underwent "additional extensive testing."
All races scheduled for this weekend would be rescheduled, the release said.
Sunday, demonstrators gathered at Santa Anita Park to protest the recent horse deaths. Local animal activists and Last Chance for Animals held the peaceful protest over the "ongoing danger to horses and riders from dangerous racing practices."
Before Eskenforadrink's death, 19 other horses had died within the last two months at the park, which prompted a two-day closure of the main track. Officials put the soil through intense testing, including ground-penetrating radar, to make sure nothing would happen again.
Officials said the horses that have died suffered a number of different fractures and at least one had a heart attack. Necropsies are being done to the horses to determine their exact causes of death.
Some said the unusually wet winter may be a factor in the deaths, while activists including PETA say the horses are overworked.
"Twenty dead horses is 20 too many and the only responsible action is for the track to close immediately to stop this spiral of deaths. The California Horse Racing Board and Santa Anita must do this now, and law enforcement must begin an immediate investigation of trainers and veterinarians to find out if injured horses were being forced to run," a statement from PETA said, in part.
The organization is also calling for the district attorney to investigate as track officials wait for results of their own investigation to see if the tragedies are connected.
On Sunday, Santa Anita Park officials said the condition of the racetrack is good, adding that 390 horses completed workouts at the track from Thursday to Saturday without injury.
Racing Thursday was already canceled because of weather and set to resume Friday until the suspension.