The message from Ayman al-Zawahri was posted on a militant website Saturday morning. In the audio message, al-Zawahri praised the violence linked to the film, including the attack on the U.S. consulate in the Libyan city of Benghazi.
An American ambassador and three others were killed in that attack.
The Cerritos man behind the amateur film, "Innocence of Muslims," is currently being held for a probation violation.
In the recording, al-Zawahri claimed Washington allowed the film's production under the pretext of freedom of expression, "but this freedom did not prevent them from torturing Muslim prisoners."
The Associated Press contributed to this report.