The 25-year-old American college student and her ex-boyfriend, Raffaele Sollecito, could be retried in the murder of Knox's roommate. Knox is not expected at the hearing.
Knox was originally convicted and spent four years in prison before being acquitted. If her acquittal is upheld, the legal battle is officially over for her.
Knox and Sollecito were arrested in 2007 shortly after 21-year-old British student Meredith Kercher's body was found in a pool of blood in her bedroom in the rented apartment she shared with Knox and others in the university town of Perugia, where they were exchange students.
Knox and Sollecito were initially convicted and given long prison sentences: 26 years for Knox, 25 for Sollecito. But in 2011 the appeals court acquitted them, criticizing the case mounted by prosecutors in the first trial.
Knox has been writing a memoir and plans to talk about it next month with ABC's Diane Sawyer.
The Associated Press contributed to this report.