Rebecca Grossman, co-founder of the Grossman Burn Foundation, pleaded not guilty to two felony counts each of murder and vehicular manslaughter with gross negligence, along with one felony count of hit-and-run driving resulting in death.
Prosecutors allege Grossman, 57, was speeding on Triunfo Canyon Road the night of Sept. 29 when she struck and killed 11-year-old Mark Iskander and his 8-year-old brother Jacob.
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The boys were crossing the street in a marked crosswalk with their parents at the time.
Grossman allegedly left the scene, but stopped shortly after her engine cut off. Investigators say speed and alcohol are believed to be a factor in the crash.
She faces 34 years to life in prison if convicted.
City News Service contributed to this report.