The announcement was made Tuesday by the San Marino institution.
Brody, who was 93 when she died last year, served as a member of Huntington's board of overseers for 20 years.
Huntington president Steven Koblik said the money will focus on the botanical gardens and will provide needed financial stability to the institution.
The Huntington received $15 million from the Brody estate in October and another $80 million last week. The income of Brody's home, costing more than $24 million, will also go to the Huntington.